TLR1 Protein (AA 26-795) (rho-1D4 tag)

N° du produit ABIN3137164

Détail du produit

Antigène: TLR1 (Toll-Like Receptor 1 (TLR1))

Type de proteíne: Recombinant

Attributs du protein: AA 26-795

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette TLR1 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: EECELIIKRP NANLTRVPKD LPLQTTTLDL SQNNISELQT SDILSLSKLR VLIMSYNRLQ YLNISVFKFN TELEYLDLSH NELKVILCHP TVSLKHLDLS FNAFDALPIC KEFGNMSQLQ FLGLSGSRVQ SSSVQLIAHL NISKVLLVLG DAYGEKEDPE SLRHVSTETL HIVFPSKREF RFLLDVSVST TIGLELSNIK CVLEDQGCSY FLRALSKLGK NLKLSNLTLN NVETTWNSFI NILQIVWHTP VKYFSISNVK LQGQLAFRMF NYSDTSLKAL SIHQVVTDVF SFPQSYIYSI FANMNIQNFT MSGTHMVHML CPSQVSPFLH VDFTDNLLTD MVFKDCRNLV RLKTLSLQKN QLKNLENIIL TSAKMTSLQK LDISQNSLRY SDGGIPCAWT QSLLVLNLSS NMLTGSVFRC LPPKVKVLDL HNNRIMSIPK DVTHLQALQE LNVASNSLTD LPGCGAFSSL SVLVIDHNSV SHPSEDFFQS CQNIRSLTAG NNPFQCTCEL RDFVKNIGWV AREVVEGWPD SYRCDYPESS RGTALRDFHM SPLSCDTVLL TVTIGATMLV LAVTGAFLCL YFDLPWYVRM LCQWTQTRHR ARHIPLEELQ RNLQFHAFVS YSGHDSAWVK NELLPNLEKD DIQICLHERN FVPGKSIVEN IINFIEKSYK SIFVLSPHFI QSEWCHYELY FAHHNLFHEG SDNLILILLA PIPQYSIPTN YHKLKTLMSR RTYLEWPTEK NKHGLFWANL RASINVKLVN QAEGTCYTQQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Tlr1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TLR1 (Toll-Like Receptor 1 (TLR1))

Autre désignation: Tlr1 (TLR1 Produits)

Synonymes: CD281 Protein, TIL Protein, TIL. LPRS5 Protein, rsc786 Protein, toll like receptor 1 Protein, toll-like receptor 1 Protein, TLR1 Protein, Tlr1 Protein

Sujet: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Acts as a coreceptor for M.tuberculosis lipoproteins LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR2 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). {ECO:0000250|UniProtKB:Q15399, ECO:0000269|PubMed:19362712}.

Poids moléculaire: 89.0 kDa Including tag.

UniProt: Q9EPQ1

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades