SPARC Protein (AA 18-303) (His tag)

N° du produit ABIN7313616

Détail du produit

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Type de proteíne: Recombinant

Attributs du protein: AA 18-303

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette SPARC protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Fonction: Recombinant Human SPARC Protein is produced by our Mammalian expression system and the target gene encoding Ala18-Ile303 is expressed with a 6His tag at the C-terminus.

Séquence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVIVDHH HHHH

Pureté: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Détail du antigène

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC (SPARC Produits)

Synonymes: ON Protein, AA517111 Protein, BM-40 Protein, hm:zeh0062 Protein, SPARC Protein, sparc Protein, secreted protein acidic and cysteine rich Protein, secreted acidic cysteine rich glycoprotein Protein, secreted protein, acidic, cysteine-rich (osteonectin) Protein, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Protein, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Protein, SPARC Protein, Sparc Protein, sparc Protein, sparc.L Protein, sparc.S Protein

ID gène: 6678

UniProt: P09486

Pathways: Autophagy