ERK1 Protein (double Mutant)

N° du produit ABIN5624588

N° du produit (Fournisseur): 009-001-gr1

Cette protéine Recombinant ERK1 est exprimée dans Insect cells (Sf9).

Aperçu rapide pour ERK1 Protein (double Mutant) (ABIN5624588)

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Type de proteíne: Recombinant

Origine: Humain

Source: Insect cells (Sf9)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Détail du produit

Attributs du protein: double Mutant

N° du produit (Fournisseur): 009-001-gr1

Fournisseur: Rockland

Fonction: ERK1 double mutant human recombinant protein

Attributs du produit: MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein

Purification: ERK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Application Note: Human ERK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical ERK1 phosphorylated T202 or Y204 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Other: Dot Blot

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Autre désignation: MAPK3

Sujet:

Synonyms: MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein

Background: ERK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Threonine 202 and Tyrosine 204. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. The inactive double mutant ERK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: P27361

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins