FCGR3B Protein (AA 17-200) (His tag,AVI tag,Biotin)

N° du produit ABIN5674595

Détail du produit

Antigène: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Type de proteíne: Recombinant

Attributs du protein: AA 17-200

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FCGR3B protéine est marqué à la His tag,AVI tag,Biotin.

Marque: PrecisionAvi

Séquence: AA 17-200

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 24.6 kDa. The protein migrates as 38-48 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Détail du antigène

Antigène: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Autre désignation: Fc gamma RIIIB / CD16b (FCGR3B Produits)

Synonymes: CD16 Protein, CD16b Protein, FCG3 Protein, FCGR3 Protein, FCR-10 Protein, FCRIII Protein, FCRIIIb Protein, FcRIII Protein, Fc fragment of IgG receptor IIIb Protein, Fc receptor, IgG, low affinity III Protein, Fc fragment of IgG receptor IIIa Protein, Fc-gamma RIII Protein, FCGR3B Protein, Fcgr3 Protein, FCGR3A Protein, FCGR3 Protein

Sujet: CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Poids moléculaire: 24.6 kDa