LILRA3 Protein (AA 24-439) (His tag,AVI tag,Biotin)

N° du produit ABIN5674602

Détail du produit

Antigène: LILRA3 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (Without TM Domain), Member 3 (LILRA3))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 24-439

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette LILRA3 protéine est marqué à la His tag,AVI tag,Biotin.

Marque: PrecisionAvi

Séquence: AA 24-439

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 48.7 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by reduced SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Détail du antigène

Antigène: LILRA3 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (Without TM Domain), Member 3 (LILRA3))

Autre désignation: LILRA3 (LILRA3 Produits)

Synonymes: CD85E Protein, HM31 Protein, HM43 Protein, ILT-6 Protein, ILT6 Protein, LIR-4 Protein, LIR4 Protein, e3 Protein, leukocyte immunoglobulin like receptor A3 Protein, LILRA3 Protein

Sujet: Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILRA3) is also known as CD85 antigen-like family member E (CD85e), immunoglobulin-like transcript 6 (ILT-6), and leukocyte immunoglobulin-like receptor 4 (LIR-4) is a protein that in humans is encoded by the LILRA3 gene located within the eukocyte receptor complex on chromosome 19q13.4. Unlike many of its family, LILRA3 lacks a transmembrane domain, which contains 4 Ig-like C2-type (immunoglobulin-like) domains. LILRA3 acts as soluble receptor for class I MHC antigens. At the same time,LILRA3 can bind both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Also,LILRA3 can bind with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

Poids moléculaire: 48.7 kDa