PRKDC Protein (AA 3747-4015) (His tag)

N° du produit ABIN5710145

Détail du produit

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Type de proteíne: Recombinant

Attributs du protein: AA 3747-4015

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PRKDC protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: EYPFLVKGGE DLRQDQRIEQ IFEVMNAILS QDAACSQRNM QLRTYRVVPM TSRLGLIEWI ENTMTLKDLL LSNMSQEEKV ANNSDPKAPI RDYKDWLMKV SGKSDAGAYV LMYSRANRTE TVVAFRRRES QVPPDLLKRA FVKMSTSPEA FLALRSHFAS SHALLCISHW LLGIGDRHLN NFMVAMETGS VIGIDFGHAF GSATQFLPVP ELMPFRLTRQ FVSLMLPMKE TGLMCTVMVH ALRAFRSCAG LLTDTMEIFV KEPSFDWKS

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Détail du antigène

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Autre désignation: PRKDC (PRKDC Produits)

Synonymes: DNA-PKcs Protein, DNAPK Protein, DNPK1 Protein, HYRC Protein, HYRC1 Protein, XRCC7 Protein, p350 Protein, DNA-PKCS Protein, dna-pkcs Protein, dnapk Protein, dnpk1 Protein, hyrc Protein, hyrc1 Protein, prkdc-A Protein, xrcc7 Protein, PRKDC Protein, AI326420 Protein, AU019811 Protein, DNAPDcs Protein, DOXNPH Protein, dxnph Protein, p460 Protein, scid Protein, slip Protein, protein kinase, DNA-activated, catalytic polypeptide Protein, protein kinase, DNA-activated, catalytic polypeptide L homeolog Protein, DNA-dependent protein kinase catalytic subunit Protein, protein kinase, DNA activated, catalytic polypeptide Protein, PRKDC Protein, prkdc.L Protein, LOC100633457 Protein, Prkdc Protein

Sujet: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artis (DCLRE1C). The assbly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism.

Poids moléculaire: 34.7 kDa

UniProt: P97313

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response