POLN Protein (AA 60-240) (His tag)

N° du produit ABIN5710850

Détail du produit

Antigène: POLN (Polymerase (DNA Directed) nu (POLN))

Type de proteíne: Recombinant

Attributs du protein: AA 60-240

Origine: Hepatitis E Virus (HEV)

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette POLN protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: EVFWNHPIQR VIHNELELYC RARSGRCLEI GAHPRSINDN PNVVHRCFLR PAGRDVQRWY TAPTRGPAAN CRRSALRGLP AADRTYCFDG FSGCNFPAET GIALYSLHDM SPSDVAEAMF RHGMTRLYAA LHLPPEVLLP PGTYRTASYL LIHDGRRVVV TYEGDTSAGY NHDVSNLRSW I

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Détail du antigène

Antigène: POLN (Polymerase (DNA Directed) nu (POLN))

Autre désignation: POLN (POLN Produits)

Synonymes: POLN Protein, POL4P Protein, DNA polymerase nu Protein, polymerase (DNA directed) nu Protein, DNA polymerase N Protein, POLN Protein, Poln Protein

Classe de substances: Viral Protein

Sujet: Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction .RNA-directed RNA polymerase plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA, probably to initiate replication ..

Poids moléculaire: 24.5 kDa

UniProt: P33424