hCoV-OC43 Spike Protein (AA 15-344, partial) (His tag)

N° du produit ABIN5714100

Détail du produit

Antigène: hCoV-OC43 Spike (HCoV-OC43 S) (Coronavirus OC43 Spike Protein (HCoV-OC43 S))

Type de proteíne: Recombinant

Attributs du protein: partial, AA 15-344

Origine: Human Coronavirus OC43 (HCoV-OC43)

Source: Levure

Purification/Conjugué: Cette hCoV-OC43 Spike protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: VIGDLKCTSD NINDKDTGPP PISTDTVDVT NGLGTYYVLD RVYLNTTLFL NGYYPTSGST YRNMALKGSV LLSRLWFKPP FLSDFINGIF AKVKNTKVIK DRVMYSEFPA ITIGSTFVNT SYSVVVQPRT INSTQDGDNK LQGLLEVSVC QYNMCEYPQT ICHPNLGNHR KELWHLDTGV VSCLYKRNFT YDVNADYLYF HFYQEGGTFY AYFTDTGVVT KFLFNVYLGM ALSHYYVMPL TCNSKLTLEY WVTPLTSRQY LLAFNQDGII FNAEDCMSDF MSEIKCKTQS IAPPTGVYEL NGYTVQPIAD VYRRKPNLPN CNIEAWLNDK

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Référence

Bhalla, Payam, Morelli, Sharma, Johnson, Thomson, Jolly, Canfarotta: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." dans: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).

Détail du antigène

Antigène: hCoV-OC43 Spike (HCoV-OC43 S) (Coronavirus OC43 Spike Protein (HCoV-OC43 S))

Autre désignation: OC43 Spike Glycoprotein (HCoV-OC43 S Produits)

Classe de substances: Viral Protein

Sujet: S1 attaches the virion to the cell mbrane by interacting with sialic acid-containing cell receptors, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell mbrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell mbranes.

Poids moléculaire: 39.6 kDa

UniProt: P36334