POLE3 Protein (AA 1-147) (His tag)

N° du produit ABIN5853016

Détail du produit

Antigène: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-147

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette POLE3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.2M NaCl, 20 % glycerol, 1  mM DTT.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Autre désignation: POLE3 (POLE3 Produits)

Synonymes: NCU04548.1 Protein, GB14966 Protein, zgc:73351 Protein, 1810034K18Rik Protein, YBL1 Protein, CHARAC17 Protein, CHRAC17 Protein, p17 Protein, DNA polymerase epsilon 3, accessory subunit Protein, polymerase (DNA directed), epsilon 3, accessory subunit Protein, DNA polymerase epsilon Protein, DNA polymerase epsilon p17 subunit Protein, DNA polymerase epsilon subunit 3 Protein, polymerase (DNA directed), epsilon 3 (p17 subunit) Protein, POLE3 Protein, pole3 Protein, NCU04548 Protein, CpipJ_CPIJ008666 Protein, Bm1_04865 Protein, Bm1_07285 Protein, CTRG_00708 Protein, LOAG_09571 Protein, LOC726923 Protein, Pole3 Protein

Sujet: POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. POLE3 has been shown to interact with SMARCA5. Recombinant human POLE3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 19kDa (170aa), confirmed by MALDI-TOF

NCBI Accession: NP_059139

UniProt: Q9NRF9

Pathways: Réparation de l'ADN