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SEMG1 Protein (AA 24-462) (His tag)
SEMG1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 85 % by SDS - PAGE
SDS
N° du produit ABIN5853026
Détail du produit
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Antigène
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SEMG1
(Semenogelin I (SEMG1))
Type de proteíne
Recombinant
Attributs du protein
AA 24-462
Origine
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Humain
Source
Toutes les sources à travers SEMG1 Protéines
Escherichia coli (E. coli)
Purification/Conjugué
Cette SEMG1 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT
Pureté
> 85 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.25 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 7.5) containing 0.5M NaCl, 10 % glycerol, 250 mM Imidazole, 0.1 mM PMSF, 1 mM DTT.
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
SEMG1
(Semenogelin I (SEMG1))
Autre désignation
SEMG1 (SEMG1 Produits )
Synonymes
SEMG1 Protein, SEMG1a Protein, CT103 Protein, SEMG Protein, SGI Protein, dJ172H20.2 Protein, RATSVPIIA Protein, SVPIIA Protein, SVS2P Protein, Svp1 Protein, Svs2 Protein, Svs2p2 Protein, BB115391 Protein, Semg1 Protein, SvsII Protein, semenogelin II Protein, semenogelin I Protein, semenogelin-2 Protein, seminal vesicle secretory protein 2 Protein, SEMG2 Protein, SEMG1 Protein, LOC100408385 Protein, Semg1 Protein, Svs2 Protein
Sujet
SEMG1 is the predominant protein in semen. The secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human SEMG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
52kDa (462aa)
NCBI Accession
NP_002998
UniProt
P04279
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