HCV 1b NS5B Protein (AA 2634-2752) (His tag)

N° du produit ABIN5853698

Détail du produit

Antigène: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Type de proteíne: Recombinant

Attributs du protein: AA 2634-2752

Origine: Hepatitis C Virus (HCV)

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette HCV 1b NS5B protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSPMGF SYDTRCFDST VTESDIRTEE AIYQCCDLDP QARVAIKSLT ERLYVGGPLT NSRGENCGYR RCRASGVLTT SCGNTLTCYI KARAACRAAG LQDCTMLVCG DDLVVICESA GVQED

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Autre désignation: HCV-NS5b (HCV NS5b Produits)

Classe de substances: Viral Protein

Sujet: Hepatitis C virus genotype 1 is a core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit and also regulates many host cellular functions such as signaling pathways and apoptosis. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Recombinant HCV(Hepatitis C Virus) NS5b protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 17.0 kDa (155aa) confirmed by MALDI-TOF

NCBI Accession: NP_671491