ATP6V1F Protein (AA 1-119) (His tag)

N° du produit ABIN5854054

Détail du produit

Antigène: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Type de proteíne: Recombinant

Attributs du protein: AA 1-119

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ATP6V1F protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate buffered saline (  pH 7.4) containing, 50 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Autre désignation: ATP6V1F (ATP6V1F Produits)

Synonymes: Atp6s14 Protein, Vma7 Protein, atp6s14 Protein, atp6v1f Protein, vatf Protein, ATP6S14 Protein, VATF Protein, 1110004G16Rik Protein, ATPase H+ transporting V1 subunit F Protein, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F S homeolog Protein, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F L homeolog Protein, ATPase, H+ transporting, lysosomal V1 subunit F Protein, Atp6v1f Protein, atp6v1f.S Protein, ATP6V1F Protein, atp6v1f.L Protein

Sujet: ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. Recombinant human ATP6V1F, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 15.8kDa (142aa) confirmed by MALDI-TOF

NCBI Accession: NP_004222

UniProt: Q16864

Pathways: Transition Metal Ion Homeostasis, Proton Transport