PGK1 Protein (AA 1-4170) (His tag)

N° du produit ABIN5854214

Détail du produit

Antigène: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-4170

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PGK1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity: > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Autre désignation: Pgk1 (PGK1 Produits)

Synonymes: MIG10 Protein, PGKA Protein, Pgk-1 Protein, Pgk Protein, PGK Protein, wu:fd59b07 Protein, wu:fj36g06 Protein, zgc:56252 Protein, zgc:77899 Protein, PGK1 Protein, phosphoglycerate kinase 1 Protein, pgk2 Protein, pgka Protein, phosphoglycerate kinase 1 Protein, phosphoglycerate kinase 1 L homeolog Protein, PGK1 Protein, Pgk1 Protein, pgk1 Protein, LOC100348124 Protein, pgk1.L Protein

Sujet: Pgk1 also known as Phosphoglycerate kinase 1 is an X-linked enzyme that plays a key role in the glycolytic pathway. Pgk1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. This protein catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant mouse Pgk1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 47.1kDa (441aa) confirmed by MALDI-TOF

NCBI Accession: NP_032854

UniProt: P09411

Pathways: Cellular Glucan Metabolic Process