ATP1B1 Protein (AA 63-303) (His tag)

N° du produit ABIN5854790

Détail du produit

Antigène: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 63-303

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette ATP1B1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 3,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Autre désignation: ATP1B1 (ATP1B1 Produits)

Synonymes: ATP1B Protein, Atp4b Protein, Atpb Protein, Atpb-1 Protein, NKbeta1 Protein, ATPBS Protein, atp1b1 Protein, atp1b1a Protein, cb710 Protein, ATPase Na+/K+ transporting subunit beta 1 Protein, ATPase, Na+/K+ transporting, beta 1 polypeptide Protein, ATPase beta chain Protein, ATP synthase CF1 beta subunit Protein, ATPase Na+/K+ transporting subunit beta 1 S homeolog Protein, ATPase, Na+/K+ transporting, beta 1b polypeptide Protein, ATP1B1 Protein, Atp1b1 Protein, atpB Protein, atp1b1.S Protein, atp1b1b Protein

Sujet: ATP1B1, also known as ATPase Na+/K+ transporting subunit beta 1, belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Recombinant human ATP1B1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 29.0kDa (250aa) 28-40KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001668

UniProt: P05026

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome