AHCY Protein (AA 1-432) (His tag)

N° du produit ABIN5854973

Détail du produit

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Type de proteíne: Recombinant

Attributs du protein: AA 1-432

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette AHCY protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSHMSDKLP YKVADIGLAA WGRKALDIAE NEMPGLMRMR EMYSASKPLK GARIAGCLHM TVETAVLIET LVALGAEVRW SSCNIFSTQD HAAAAIAKAG IPVFAWKGET DEEYLWCIEQ TLHFKDGPLN MILDDGGDLT NLIHTKYPQL LSGIRGISEE TTTGVHNLYK MMSNGILKVP AINVNDSVTK SKFDNLYGCR ESLIDGIKRA TDVMIAGKVA VVAGYGDVGK GCAQALRGFG ARVIITEIDP INALQAAMEG YEVTTMDEAC KEGNIFVTTT GCVDIILGRH FEQMKDDAIV CNIGHFDVEI DVKWLNENAV EKVNIKPQVD RYWLKNGRRI ILLAEGRLVN LGCAMGHPSF VMSNSFTNQV MAQIELWTHP DKYPVGVHFL PKKLDEAVAE AHLGKLNVKL TKLTEKQAQY LGMPINGPFK PDHYRY

Pureté: > 90 % by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-Hcl buffer (  pH 8.0) containing 40 % glycerol, 0.2M NaCl, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Autre désignation: Ahcy (AHCY Produits)

Synonymes: sahh Protein, PSPTO5068 Protein, Ahcy Protein, SAHH Protein, adoHcyase Protein, ahcy Protein, AA987153 Protein, AL024110 Protein, CuBP Protein, D150 Protein, cb1079 Protein, hm:zeh1173 Protein, hm:zeh1364 Protein, wu:fj67b02 Protein, adenosylhomocysteinase Protein, Adenosylhomocysteinase Protein, S-adenosylhomocysteine hydrolase Protein, adenosylhomocysteinase S homeolog Protein, AHCY Protein, ahcy Protein, ahcY Protein, MMAH_RS02605 Protein, Srot_2217 Protein, Ndas_3755 Protein, Deba_0936 Protein, Palpr_2392 Protein, Calni_0569 Protein, LOC100282150 Protein, Intca_2510 Protein, Marky_0392 Protein, Desac_1394 Protein, Tc00.1047053511229.50 Protein, Tc00.1047053511589.200 Protein, Tb11.01.1350 Protein, LMJF_36_3910 Protein, MCYG_06244 Protein, Ahcy Protein, ahcy.S Protein

Sujet: Ahcy, also known as Adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-sdenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant mouse Ahcy, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 50.2 kDa (456aa) confirmed by MALDI-TOF

NCBI Accession: NP_057870

UniProt: P50247