Glypican 3 Protein (GPC3) (AA 25-559) (His tag,AVI tag,Biotin)

N° du produit ABIN5954920

Détail du produit

Antigène: Glypican 3 (GPC3)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 25-559

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Glypican 3 protéine est marqué à la His tag,AVI tag,Biotin.

Séquence: AA 25-559

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Pureté: >85 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Référence

Berman, Kelada, Gutsche, Natarajan, Swenson, Fu, Hong, Ho, Choyke, Escorcia: "In Vitro Performance of Published Glypican 3-Targeting Peptides TJ12P1 and L5 Indicates Lack of Specificity and Potency." dans: Cancer biotherapy & radiopharmaceuticals, (2019) (PubMed).

Détail du antigène

Antigène: Glypican 3 (GPC3)

Autre désignation: Glypican 3 (GPC3 Produits)

Synonymes: GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein

Sujet: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Poids moléculaire: 38.1 kDa and 26.4 kDa

NCBI Accession: NP_004475

Pathways: Glycosaminoglycan Metabolic Process