TNFRSF12A Protein (AA 24-126) (His tag,GST tag)

N° du produit ABIN6239776

Détail du produit

Antigène: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Activité biologique: Active

Attributs du protein: AA 24-126

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette TNFRSF12A protéine est marqué à la His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His and GST Tag

Pureté: > 80 %

Biological Activity Comment: TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TNFRSF12A with TNFa.

Information d'application

Indications d'application: Isoelectric Point: 6.7

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Abstract: TNFRSF12A Produits

Synonymes: CD266 Protein, FN14 Protein, TWEAKR Protein, AI255180 Protein, C87282 Protein, Fn14 Protein, HPIP Protein, TWEAK-R Protein, TweakR Protein, TNF receptor superfamily member 12A Protein, tumor necrosis factor receptor superfamily, member 12a Protein, TNFRSF12A Protein, Tnfrsf12a Protein

Sujet: Alternative Names: CD266, TNFRSF12-A, FN14, TWEAKR, Tumour Necrosis Factor Related Weak Inducer Of Apoptosis, Fibroblast growth factor-inducible immediate-early response protein 14

Poids moléculaire: 44kDa

UniProt: Q9NP84

Pathways: Apoptose, Regulation of Cell Size