GZMM Protein (AA 26-257) (His tag)

N° du produit ABIN5665807

Détail du produit

Antigène: GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))

Activité biologique: Active

Attributs du protein: AA 26-257

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette GZMM protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 90 %

Biological Activity Comment: GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2x107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin. Normal Heated 0 4 4 GZMM (μM) Ezrin Actin Figure 1. GZMM cleaves Ezrin

Information d'application

Indications d'application: Isoelectric Point: 10.3

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))

Autre désignation: Granzyme M (GZMM) (GZMM Produits)

Synonymes: LMET1 Protein, MET1 Protein, Lmet1 Protein, MMET-1 Protein, granzyme M Protein, granzyme M (lymphocyte met-ase 1) Protein, LOC100136381 Protein, GZMM Protein, Gzmm Protein

Sujet: Alternative Names: GZM-M, LMET1, MET1, Hu-Met-1, Met-ase, Lymphocyte Met Ase 1, Met-1 serine protease, Natural killer cell granular protease

Poids moléculaire: 26kDa

UniProt: P51124