IL17RC Protein (AA 257-477) (His tag)

N° du produit ABIN6239824

Détail du produit

Antigène: IL17RC (Interleukin 17 Receptor C (IL17RC))

Activité biologique: Active

Attributs du protein: AA 257-477

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IL17RC protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: IL17RC (Interleukin-17 receptor C) is a receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. This protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. A binding ELISA assay was conducted to detect the association of IL17RC with IL17RA. Briefly, IL17RC were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RC with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17RC with IL17RA.

Information d'application

Indications d'application: Isoelectric Point: 5.8

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL17RC (Interleukin 17 Receptor C (IL17RC))

Abstract: IL17RC Produits

Synonymes: IL17-RL Protein, IL17RL Protein, 1110025H02Rik Protein, Gm19850 Protein, IL-17RC Protein, IL17-RC Protein, Il17rl Protein, interleukin 17 receptor C Protein, Il17rc Protein, IL17RC Protein

Sujet: Alternative Names: IL17-RL, IL17R-C, IL17Rhom, Interleukin-17 receptor homolog, Interleukin-17 receptor-like protein, ZcytoR14

Poids moléculaire: 26kDa

UniProt: Q8NAC3