Cathepsin L2 Protein (CTSL2) (AA 64-334) (His tag)

N° du produit ABIN6239914

Détail du produit

Antigène: Cathepsin L2 (CTSL2)

Activité biologique: Active

Attributs du protein: AA 64-334

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Cathepsin L2 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CTSV with RB1.

Information d'application

Indications d'application: Isoelectric Point: 8.8

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: Cathepsin L2 (CTSL2)

Autre désignation: Cathepsin V (CTSV) (CTSL2 Produits)

Synonymes: CATL2 Protein, CTSL2 Protein, CTSU Protein, CTSL Protein, CTSL1 Protein, ctsl2 Protein, LOC100232421 Protein, cathepsin V Protein, cathepsin V L homeolog Protein, cathepsin L Protein, CTSV Protein, ctsv.L Protein, ctsv Protein, CTSL Protein, Ctsv Protein

Sujet: Alternative Names: CTS-L2, CTSL2, CATL2, CTSU, CTS-V, Cathepsin L2

Poids moléculaire: 32kDa

UniProt: O60911

Pathways: Toll-Like Receptors Cascades