IL-27 Protein (AA 21-229, AA 26-243) (Fc Tag)

N° du produit ABIN6253326

Détail du produit

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Type de proteíne: Recombinant

Attributs du protein: AA 21-229, AA 26-243

Origine: Humain

Source: CHO Cells

Purification/Conjugué: Cette IL-27 protéine est marqué à la Fc Tag.

Fonction: IL-27 (human):Fc (human) (rec.) (non-lytic)

Specificité: The human IL-27 complex composed of the Ebi3 subunit (aa 21-229) and the p28 subunit (aa 26-243) is fused through a polypeptide linker to the Fc portion of a mutant human IgG1.

Attributs du produit: Protein. The human IL-27 complex composed of the Ebi3 subunit (aa 21-229) and the p28 subunit (aa 26-243) is fused through a polypeptide linker to the Fc portion of a mutant human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an antiinflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naie CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction.

Pureté: >98 % (SDS-PAGE)

niveau d'endotoxine: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Bioactivity was measured in a cell proliferation assay using anti-CD3 activated human peripheral mononuclear cells.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Détail du antigène

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Autre désignation: IL-27 (IL27 Produits)

Synonymes: IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein

Sujet:

Alternate Names/Synonyms: Interleukin-27

Product Description: Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an antiinflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naie CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction.

NCBI Accession: NP_663634