IL-27 Protein (AA 21-229) (His tag)
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- Antigène Voir toutes IL-27 (IL27) Protéines
- IL-27 (IL27) (Interleukin 27 (IL27))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 21-229
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Origine
- Humain
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Source
- CHO Cells
- Purification/Conjugué
- Cette IL-27 protéine est marqué à la His tag.
- Fonction
- Human IL-27 Protein
- Séquence
- Arg21-Lys229 (IL-27B) & Phe29-Pro243 (IL-27A)
- Specificité
- Uni-Prot: Q14213 (IL-27B), Q8NEV9 (IL-27A)
- Attributs du produit
- Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A).
- Pureté
- > 95 % as determined by Tris-Bis PAGE
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- IL-27 (IL27) (Interleukin 27 (IL27))
- Autre désignation
- IL-27 (IL27 Produits)
- Synonymes
- IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein
- Sujet
- Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
- Poids moléculaire
- 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.
- UniProt
- Q14213
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