EGLN3 Protein (His tag)

N° du produit ABIN1098639

Détail du produit

Antigène: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette EGLN3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Purification: purified by using conventional chromatography.

Information d'application

Commentaires:

Synonyms: Egl nine homolog 3, HIFPH3, PHD3

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5 MM DTT, 2 mM EDTA

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Autre désignation: EGLN3 (EGLN3 Produits)

Synonymes: zgc:77019 Protein, wu:fj78a08 Protein, EGLN3 Protein, phd3 Protein, PHD-3 Protein, PHD3 Protein, SM-20 Protein, 2610021G09Rik Protein, AI505553 Protein, AI648162 Protein, Hif-p4h-3 Protein, Phd3 Protein, HIFP4H3 Protein, HIFPH3 Protein, egl-9 family hypoxia-inducible factor 3 Protein, egl-9 family hypoxia inducible factor 3 Protein, egl-9 family hypoxia-inducible factor 3 L homeolog Protein, egln3 Protein, EGLN3 Protein, egln3.L Protein, Egln3 Protein

Sujet: EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 29.8 kDa (263aa) confirmed by MALDI-TOF

NCBI Accession: NP_071356

Pathways: Positive Regulation of Endopeptidase Activity