YOD1 Protein (His tag)

N° du produit ABIN1098573

Détail du produit

Antigène: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette YOD1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Pureté: > 90 % by SDS - PAGE

Information d'application

Commentaires:

Synonyms: Ubiquitin thioesterase OTU1, DUBA8, OTUD2, PRO0907, RP11-164O23.1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 30% glycerol, 1 MM DTT

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Autre désignation: YOD1 (YOD1 Produits)

Synonymes: DUBA8 Protein, OTUD2 Protein, PRO0907 Protein, hshin7 Protein, wu:fe01a10 Protein, zgc:112182 Protein, 9930028C20Rik Protein, Hshin7 Protein, YOD1 deubiquitinase Protein, YOD1 deubiquitinase L homeolog Protein, YOD1 Protein, Yod1 Protein, yod1.L Protein, yod1 Protein

Sujet: YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates. Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 40.7 kDa (371aa) confirmed by MALDI-TOF

NCBI Accession: NP_061036

Pathways: ER-Nucleus Signaling