HCV NS5a Protein (AA 2210-2306) (His tag)

N° du produit ABIN6387451

Détail du produit

Antigène: HCV NS5a (Hepatitis C Virus NS5a (HCV NS5a))

Type de proteíne: Recombinant

Attributs du protein: AA 2210-2306

Origine: Hepatitis C Virus (HCV)

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette HCV NS5a protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESEN KVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.2M NaCl, 40 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: HCV NS5a (Hepatitis C Virus NS5a (HCV NS5a))

Autre désignation: HCV-NS5a (HCV NS5a Produits)

Classe de substances: Viral Protein

Sujet: NS5A is a component of the replication complex involved in RNA-binding. Its interaction with Human VAPB may target the viral replication complex to vesicles. This protein down-regulates viral IRES translation initiation and mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. NS5a seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication. Recombinant HCV(Hepatitis C Virus) NS5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 13.4 kDa (118aa) confirmed by MALDI-TOF

NCBI Accession: NP_671491