PDIA6 Protein (AA 20-440) (His tag)

N° du produit ABIN6387616

Détail du produit

Antigène: PDIA6 (Protein Disulfide Isomerase Family A, Member 6 (PDIA6))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 20-440

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PDIA6 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing, 50  mM NaCl, 2  mM DTT and 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PDIA6 (Protein Disulfide Isomerase Family A, Member 6 (PDIA6))

Autre désignation: PDIA6 (PDIA6 Produits)

Synonymes: 1700015E05Rik Protein, AL023058 Protein, C77895 Protein, CaBP5 Protein, P5 Protein, Txndc7 Protein, ERP5 Protein, TXNDC7 Protein, CaBP1 Protein, pdi-p5 Protein, erp5 Protein, pdia6 Protein, pdip5 Protein, txndc7 Protein, protein disulfide isomerase family A member 6 Protein, protein disulfide isomerase associated 6 Protein, protein disulfide isomerase family A, member 6 Protein, protein disulfide isomerase family A member 6 L homeolog Protein, PDIA6 Protein, Pdia6 Protein, pdia6.L Protein

Sujet: PDIA6 is a member of the protein disulfide isomerase (PDI). PDI is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. PDIA6 function as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Recombinant human PDIA6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 48.5 kDa (442aa), confirmed by MALDI-TOF

NCBI Accession: NP_005733

UniProt: Q15084

Pathways: ER-Nucleus Signaling, Cell RedoxHomeostasis