CSTA Protein (AA 1-98) (His tag)

N° du produit ABIN6387718

Détail du produit

Antigène: CSTA (Cystatin A (Stefin A) (CSTA))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-98

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CSTA protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CSTA (Cystatin A (Stefin A) (CSTA))

Autre désignation: CSTA (CSTA Produits)

Synonymes: AREI Protein, STF1 Protein, STFA Protein, CST Protein, CSTA Protein, ATCYSA Protein, FL3-27 Protein, T20B5.8 Protein, T20B5_8 Protein, cystatin A Protein, cystatin A Protein, cystatin A1 Protein, cystatin-A Protein, CSTA Protein, Csta1 Protein, Csta Protein, CYSA Protein, LOC100725920 Protein

Sujet: CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies Recombinant human CSTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 13.1 kDa (118aa) confirmed by MALDI-TOF

NCBI Accession: NP_005204

UniProt: P01040

Pathways: Response to Water Deprivation