PGK2 Protein (AA 1-417) (His tag)

N° du produit ABIN5854495

Détail du produit

Antigène: PGK2 (Phosphoglycerate Kinase 2 (PGK2))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-417

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PGK2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 1  mM DTT, 0.1M NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PGK2 (Phosphoglycerate Kinase 2 (PGK2))

Autre désignation: PGK2 (PGK2 Produits)

Synonymes: PGKB Protein, PGKPS Protein, dJ417L20.2 Protein, Pgk-2 Protein, Tcp-2 Protein, PGK2 Protein, phosphoglycerate kinase 2 Protein, PGK2 Protein, Pgk2 Protein, cbbK Protein

Sujet: PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 46.9 kDa (437aa), confirmed by MALDI-TOF

NCBI Accession: NP_620061

UniProt: P07205