PGAM1 Protein (AA 1-254) (His tag)

N° du produit ABIN6387877

Détail du produit

Antigène: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-254

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PGAM1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Autre désignation: PGAM1 (PGAM1 Produits)

Synonymes: PGAM-B Protein, PGAMA Protein, Pgmut Protein, MGC81450 Protein, 2310050F24Rik Protein, Pgam-1 Protein, pgam1 Protein, wu:fb81h05 Protein, fd15c02 Protein, pgam1lb Protein, wu:fd15c02 Protein, zgc:63722 Protein, phosphoglycerate mutase 1 Protein, phosphoglycerate mutase 1 L homeolog Protein, phosphoglycerate mutase 1a Protein, phosphoglycerate mutase 1b Protein, PGAM1 Protein, Pgam1 Protein, pgam1.L Protein, pgam1 Protein, pgam1a Protein, pgam1b Protein

Sujet: PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 31.4 kDa (278aa) confirmed by MALDI-TOF

NCBI Accession: NP_075907

UniProt: Q9DBJ1

Pathways: Regulation of Carbohydrate Metabolic Process