DUSP18 Protein (AA 1-188) (His tag)

N° du produit ABIN6388006

Détail du produit

Antigène: DUSP18 (Dual Specificity Phosphatase 18 (DUSP18))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-188

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette DUSP18 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 300 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 40 % glycerol, 0.1  mM PMSF, 1  mM EDTA

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: DUSP18 (Dual Specificity Phosphatase 18 (DUSP18))

Autre désignation: DUSP18 (DUSP18 Produits)

Synonymes: MGC131167 Protein, DSP18 Protein, DUSP20 Protein, LMWDSP20 Protein, bK963H5.1 Protein, 4930527G07Rik Protein, AI430870 Protein, BB028064 Protein, dual specificity phosphatase 18 Protein, dual specificity phosphatase 18 L homeolog Protein, DUSP18 Protein, dusp18.L Protein, Dusp18 Protein

Sujet: Dual specificity phosphatase 18, also known as DUSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DUSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DUSP18 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 23.6 kDa (212aa) confirmed by MALDI-TOF

NCBI Accession: NP_689724

UniProt: Q8NEJ0