CST3 Protein (AA 21-140) (His tag)

N° du produit ABIN5777481

Détail du produit

Antigène: CST3 (Cystatin C (CST3))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 21-140

Origine: Souris

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette CST3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ATPKQGPRML GAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNL TDCPFHDQPH LMRKALCSFQ IYSVPWKGTH SLTKFSCKNA HHHHHH

Pureté: > 95 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CST3 (Cystatin C (CST3))

Autre désignation: Cystatin-C (CST3 Produits)

Synonymes: armd11 Protein, fb51d07 Protein, wu:fb24g06 Protein, wu:fb51d07 Protein, wu:fc55f03 Protein, zgc:136227 Protein, ARMD11 Protein, CysC Protein, CYSC Protein, cystatin C Protein, cystatin C L homeolog Protein, cystatin C (amyloid angiopathy and cerebral hemorrhage) Protein, cystatin 3 Protein, CST3 Protein, cst3.L Protein, cst3 Protein, cystatin 3 Protein, Cst3 Protein

Sujet: CST3, also known as Cystatin-C is a member of family 2 of the cystatin superfamily. This protein is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy. Recombinant Mouse CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 14.2kDa (126aa) confirmed by MALDI-TOF 13.5-18KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_034106

UniProt: P21460