Chromogranin A Protein (CHGA) (AA 19-457) (His tag)

N° du produit ABIN7529384

Détail du produit

Antigène: Chromogranin A (CHGA)

Type de proteíne: Recombinant

Attributs du protein: AA 19-457

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Chromogranin A protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADLLPVNSPM NKGDTEVMKC IVEVISDTLS KPSPMPVSQE CFETLRGDER ILSILRHQNL LKELQDLALQ GAKERAHQQK KHSGFEDELS EVLENQSSQA ELKEAVEEPS SKDVMEKRED SKEAEKSGEA TDGARPQALP EPMQESKAEG NNQAPGEEEE EEEEATNTHP PASLPSQKYP GPQAEGDSEG LSQGLVDREK GLSAEPGWQA KREEEEEEEE EAEAGEEAVP EEEGPTVVLN PHPSLGYKEI RKGESRSEAL AVDGAGKPGA EEAQDPEGKG EQEHSQQKEE EEEMAVVPQG LFRGGKSGEL EQEEERLSKE WEDSKRWSKM DQLAKELTAE KRLEGQEEEE DNRDSSMKLS FRARAYGFRG PGPQLRRGWR PSSREDSLEA GLPLQVRGYP EEKKEEEGSA NRRPEDQELE SLSAIEAELE KVAHQLQALR RGHHHHHH

Pureté: > 85 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 20 % glycerol, 0.1  mM PMSF

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Chromogranin A (CHGA)

Autre désignation: CHGA (CHGA Produits)

Synonymes: CGA Protein, CG-ALPHA Protein, FSHA Protein, GPHA1 Protein, GPHa Protein, HCG Protein, LHA Protein, TSHA Protein, ChrA Protein, fj05f09 Protein, si:dkey-177p2.2 Protein, wu:fj05f09 Protein, zgc:101749 Protein, zgc:56075 Protein, CHGA Protein, cgA Protein, chga Protein, chromogranin A Protein, glycoprotein hormones, alpha polypeptide Protein, uncharacterized CHGA Protein, chromogranin A S homeolog Protein, CHGA Protein, CGA Protein, Chga Protein, chga Protein, chga.S Protein

Sujet: CHGA, also known as chromogranin-A isoform 1 preproprotein, is a member of neuroendocrine secretory proteins of the chromogranin / secretogranin family. It is a precursor of several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. This peptide negatively regulates the neuroendocrine function of autocrine or nearby cells (paracrine). This protein induces and promotes the production of secretory granules containing insulin in pancreatic islet beta cells. Recombinant human CHGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 50kDa (448aa) 50-70kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_001266

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling