PPM1G Protein (AA 1-546) (His tag)

N° du produit ABIN6388210

Détail du produit

Antigène: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Type de proteíne: Recombinant

Attributs du protein: AA 1-546

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PPM1G protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

Pureté: > 85 % by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 0.1M NaCl , 0.1  mM PMSF

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Autre désignation: PPM1G (PPM1G Produits)

Synonymes: AI385652 Protein, Fin13 Protein, PP2CG Protein, PP2CGAMMA Protein, PPP2CG Protein, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent 1G Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1G Protein, Ppm1g Protein, PPM1G Protein

Sujet: PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that is negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. Recombinant human PPM1G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 61.4 kDa (566aa)

NCBI Accession: NP_817092

UniProt: O15355