Hexokinase 2 Protein (HK2) (AA 1-546) (His tag)

N° du produit ABIN7281354

Détail du produit

Antigène: Hexokinase 2 (HK2)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-546

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Hexokinase 2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 40,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol, 0.1M NaCl , 0.1  mM PMSF

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Hexokinase 2 (HK2)

Autre désignation: Hexokinase2 (HK2 Produits)

Synonymes: HKII Protein, HXK2 Protein, AI642394 Protein, hexokinase-2 Protein, fi09d05 Protein, fi17h06 Protein, wu:fi09d05 Protein, wu:fi17h06 Protein, wu:fi31b04 Protein, zgc:55926 Protein, HK2 Protein, ARABIDOPSIS THALIANA HEXOKINASE 2 Protein, ATHXK2 Protein, F6F22.11 Protein, F6F22_11 Protein, HEXOKINASE 2 Protein, hexokinase 2 Protein, StHK2 Protein, hexokinase 2 Protein, hexokinase-2 Protein, hexokinase 2 L homeolog Protein, HK2 Protein, Hk2 Protein, hk2 Protein, HXK2 Protein, LOC100032849 Protein, hk2.L Protein, hxk2 Protein, LOC102577690 Protein

Sujet: Hexokinase2 is one of four highly homologous hexokinase isoforms in mammalian cells. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Recombinant human Hexokinase2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 104.5 kDa (937aa)

NCBI Accession: NP_000180

UniProt: P52789

Pathways: Signalisation PI3K-Akt, Carbohydrate Homeostasis, L'effet Warburg