EGF Protéine

N° du produit ABIN6699794

N° du produit (Fournisseur): 009-001-c26

Cette protéine Recombinant EGF est exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 2+ publications.

Aperçu rapide pour EGF Protéine (ABIN6699794)

Antigène: EGF (Epidermal Growth Factor (EGF))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: 95,00%

Détail du produit

N° du produit (Fournisseur): 009-001-c26

Fournisseur: Rockland

Fonction: EGF Human Recombinant Epidermal Growth Factor

Purification: Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.

niveau d'endotoxine: Measured by LAL is 0.5 EU/μg protein.

Biological Activity Comment: The ED50, calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells, is 30-40 pg/ml.

Information d'application

Indications d'application:

Application Note: EGF Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-EGF in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5.0 mL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Référence

Khan, Park, Fischer, Dong, Lungjangwa, Jimenez, Casalena, Chew, Dietmann, Auld, Jaenisch, Theunissen: "Probing the signaling requirements for naive human pluripotency by high-throughput chemical screening." dans: Cell reports, Vol. 35, Issue 11, pp. 109233, (2022) (PubMed).

Dong, Beltcheva, Gontarz, Zhang, Popli, Fischer, Khan, Park, Yoon, Xing, Kommagani, Wang, Solnica-Krezel, Theunissen: "Derivation of trophoblast stem cells from naïve human pluripotent stem cells." dans: eLife, Vol. 9, (2021) (PubMed).

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF

Sujet:

Synonyms: Epidermal growth factor, Urogastrone

Background: EGF (Epidermal Growth Factor) protein, of the superfamily with the same name, is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. It is a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4 (HOMG4). Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants. This protein exists in the membrane as a single-pass type I membrane protein, and contains 9 EGF-like domains as well as 9 LDL-receptor class B repeats. It is significantly expressed in kidney, salivary gland, cerebrum and prostate.

UniProt: P01133

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation