EGF Protéine

N° du produit ABIN6699799

N° du produit (Fournisseur): 010-001-c26-1000

Cette protéine Recombinant EGF est produite dans Escherichia coli (E. coli).

Aperçu rapide pour EGF Protéine (ABIN6699799)

Antigène: EGF (Epidermal Growth Factor (EGF))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 97,00%

Détail du produit

N° du produit (Fournisseur): 010-001-c26-1000

Fournisseur: Rockland

Fonction: Mouse Epidermal Growth Factor Recombinant Protein

Purification: Epidermal Growth Factor purity was determined to be greater than 97% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 0.1 ng/mL.

Information d'application

Indications d'application:

Application Note: Epidermal Growth Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Epidermal Growth Factor in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 1.0 mL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: Egf

Sujet:

Synonyms: Urogastrone, URG

Background: Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4. Recombinant mouse EGF is a non-glycosylated protein, containing 54 amino acids, including 3 intra-molecular disulfide-bonds, with a molecular weight of 6.2 kDa.

UniProt: P01132

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation