SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)

N° du produit ABIN6952454

Détail du produit

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Nucleocapsid protéine est marqué à la His tag.

Séquence: AA 1-419

Attributs du produit: 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C5227) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # YP_009724397.2). This protein carries a polyhistidine tag at the C-terminus.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.

Référence

Sasisekharan, Pentakota, Jayaraman, Tharakaraman, Wogan, Narayanasami: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).

Supekar, Shajahan, Gleinich, Rouhani, Heiss, Chapla, Moremen, Azadi: "Variable post-translational modifications of SARS-CoV-2 nucleocapsid protein." dans: Glycobiology, (2021) (PubMed).

Qian, Lei, Patel, Lee, Monaghan-Nichols, Xin, Qiu, Fu: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." dans: bioRxiv : the preprint server for biology, (2021) (PubMed).

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." dans: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Rudberg, Havervall, Månberg, Jernbom Falk, Aguilera, Ng, Gabrielsson, Salomonsson, Hanke, Murrell, McInerney, Olofsson, Andersson, Hellström, Bayati, Bergström, Pin, Sjöberg, Tegel, Hedhammar et al.: "SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden. ..." dans: Nature communications, Vol. 11, Issue 1, pp. 5064, (2020) (PubMed).

Détail du antigène

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Autre désignation: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Produits)

Synonymes: nucleocapsid phosphoprotein Protein, N Protein

Classe de substances: Viral Protein

Sujet: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Poids moléculaire: 47.3 kDa