EGF Protein (AA 971-1023)

N° du produit ABIN7122799

Protéine Recombinant EGF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour EGF Protein (AA 971-1023) (ABIN7122799)

Antigène: EGF (Epidermal Growth Factor (EGF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Pureté: Greater than 90 % as determined by SDS-PAGE.

Détail du produit

Attributs du protein: AA 971-1023

Fonction: Recombinant Human EGF

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.01 EU/μg as determined by LAL test.

Biological Activity Comment: Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.5 ng/mL.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5 % trehalose, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Date de péremption: 12 months

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF

Sujet: Beta urogastrone, beta-urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, Pro epidermal growth factor, URG, Urogastrone

Poids moléculaire: 5.7 kDa

UniProt: P01133

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation