NUCB2 Protein (full length) (rho-1D4 tag)

N° du produit ABIN7126038

Détail du produit

Antigène: NUCB2 (Nucleobindin 2 (NUCB2))

Type de proteíne: Recombinant

Attributs du protein: full length

Origine: CHO cells

Source: Cellules d'insectes

Purification/Conjugué: Cette NUCB2 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Séquence: MRWKIIQLQY CFLLVPCMLT ALEAVPIDVD KTKVHNTEPV ESARIDPPDT GLYYDEYLKQ VIDVLETDQH FREKLQKADI EEIRSGRLSK ELDLVSHHVR TKLDELKRQE VARLRMLIKA KLDSLQDTGM NHHLLLKQFE HLNHQNPDTF ESSDLDMLIK AATADLEQYD RTRHEEFKKY EMMKEHERRE YLKTLNEEKR KEEESKFEEM KRKHENHPKV NHPGSKDQLK EVWEEADGLD PNDFDPKTFF KLHDVNNDGF LDEQELEALF TRELEKVYDP RNAEDDMIEM EEERLRMREH VMNEIDNNKD RLVTLEEFLR ATEKKEFLEP DSWETLGQQQ LFTEEELKEY ESIIAMQENE LKKRADELQK QKEELQRQHD HLEAQKQEYQ QAVQQLEHKK FQQGIAPSGP AGELEFKPRM
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Nucleobindin-2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Classe de qualité: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NUCB2 (Nucleobindin 2 (NUCB2))

Autre désignation: Nucleobindin-2 (NUCB2 Produits)

Synonymes: nefa Protein, MGC97715 Protein, NUCB2 Protein, nucleobindin-2 Protein, NEFA Protein, AI607786 Protein, Calnuc Protein, Nefa Protein, Nesfatin-1 Protein, p54 Protein, nucleobindin 2 Protein, nucleobindin 2 L homeolog Protein, nucb2 Protein, NUCB2 Protein, Nucb2 Protein, nucb2.L Protein

UniProt: G3IF52