TUBA1A Protein (full length) (rho-1D4 tag)

N° du produit ABIN7126040

Détail du produit

Antigène: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Type de proteíne: Recombinant

Attributs du protein: full length

Origine: CHO cells

Source: Cellules d'insectes

Purification/Conjugué: Cette TUBA1A protéine est marqué à la rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)

Séquence: MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Tubulin alpha-1A chain (Alpha-tubulin 2) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Classe de qualité: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Autre désignation: Tubulin alpha-1A chain (Alpha-tubulin 2) (Tuba1a Produits)

Synonymes: Tuba1 Protein, B-ALPHA-1 Protein, LIS3 Protein, TUBA3 Protein, Tuba-1 Protein, TUBA3D Protein, TUBA3E Protein, wu:fb37a10 Protein, 1t Protein, ALPHA 84C Protein, Alpha-Tubulin84B Protein, BEST:LD32507 Protein, CG1913 Protein, DM1alpha Protein, DTA1 Protein, Dmel\CG1913 Protein, T Protein, TBA1_DROME Protein, Talpha1 Protein, Tub Protein, Tub 84B Protein, Tub1C Protein, Tuba84B Protein, Tubalpha1 Protein, a-tub84B Protein, a1t Protein, aTub84B Protein, alpha-Tub Protein, alpha-Tub84B Protein, alpha-tub Protein, alpha-tub84B Protein, alpha-tubulin Protein, alpha-tubulin 84B Protein, alpha1 Protein, alpha1t Protein, alpha1tub Protein, alpha1tub84B Protein, alpha84B Protein, alphaT Protein, alphaTUB Protein, alphaTUB84B Protein, alphaTub Protein, alphaTub1 Protein, alphaTub84 Protein, alphat-1 Protein, alphatub Protein, alphatub84B Protein, anon-EST:Liang-1.59 Protein, anon-EST:Liang-2.30 Protein, chr3R:2914276..2914416 Protein, clone 1.59 Protein, clone 2.30 Protein, l(3)84Bd Protein, l(3)g3 Protein, ms(3)nc33 Protein, nc33 Protein, tub Protein, tubA84B Protein, tubalpha1 Protein, tubulin Protein, TUBA1A Protein, Tuba1a Protein, Tuba2 Protein, alphatub84 Protein, b-alpha-1 Protein, lis3 Protein, tuba1a Protein, tuba1a-a Protein, tuba1a-b Protein, tuba3 Protein, tubulin, alpha 1A Protein, tubulin alpha 1a Protein, tubulin, alpha 1a Protein, tubulin alpha-1B chain Protein, tubulin alpha-1A chain Protein, tubulin alpha-3 chain-like Protein, alpha-Tubulin at 84B Protein, tubulin alpha 1a L homeolog Protein, Tuba1a Protein, TUBA1A Protein, LOC608147 Protein, LOC100392817 Protein, LOC425049 Protein, tuba1a Protein, alphaTub84B Protein, LOC100051840 Protein, LOC100340483 Protein, LOC100726777 Protein, tuba1a.L Protein

Sujet: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

UniProt: P68362

Pathways: Dynamique des Microtubules, M Phase