MGEA5 Protein (full length) (rho-1D4 tag)
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- Antigène Voir toutes MGEA5 Protéines
- MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))
- Type de proteíne
- Recombinant
- Attributs du protein
- full length
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Origine
- CHO cells
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette MGEA5 protéine est marqué à la rho-1D4 tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)
- Séquence
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MAGCRLWVSL LLAAALASSA TAVWPWPQYI QTYHRRYTLY PNNFQFRYHA ASAAQAGCVV LDEAFRRYRN LLFGSGSWPR PSFSRKQLIL GKNVLVVSVI TAECNEFPNL ESVENYTLTI NDDQCLLVSE TVWGALRGLE TFSQLVWKSA EGTFFINKTK IKDFPRFPHR GILLDTSRHY LPLSSILDTL DVMAYNKFNV FHWHLVDDSS FPYESFTFPE LTRKGSYNPV THIYTAQDVK EVIEYARLRG IRVLAEFDTP GHTLSWGPGV PGLLTPCYSG SRPSGTFGPV NPSLNSTYDF MSTFFLEISS VFPDFYLHLG GDEVDFTCWR SNPNIEAFMK KKGFSDFKQL ESFYIQTLLD IVSDYDKGYV VWQEVFDNKV KVRPDTIIQV WREEIPVDYM KEMEEITKAG FRALLSAPWY LNRVTYGPDW KDMYKVEPLA FHGTSEQKGL VIGGEACMWG EYVDSTNLVP RLWPRAGAIA ERLWSSNLTT NMDFAFKRLS HFRCEMLRRG VQAQPISVGY CEQEFEQT
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Beta-hexosaminidase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- Classe de qualité
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product MGEA5 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))
- Autre désignation
- Beta-hexosaminidase (MGEA5 Produits)
- Synonymes
- MEA5 Protein, NCOAT Protein, OGA Protein, 2810009A20Rik Protein, 4833427O07Rik Protein, 5830447M11Rik Protein, AA408215 Protein, Hy5 Protein, Ncoat Protein, mKIAA0679 Protein, O-GLCNACASE Protein, cytochrome c oxidase subunit VIc Protein, meningioma expressed antigen 5 (hyaluronidase) Protein, COX6C Protein, MGEA5 Protein, Mgea5 Protein
- UniProt
- G3H3P8
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process
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