CD73 Protein (His tag)

N° du produit ABIN7319774

Détail du produit

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette CD73 protéine est marqué à la His tag.

Fonction: Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (P21589, C-6His)

Séquence: Trp27-Lys547

Attributs du produit: Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.

Pureté: >95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human CD73-His(Cat#CW84) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#NC034). The ED50 of Anti-Human CD73 mAb(Cat#NC034) is 7.8 ng/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20 % Glycerol, pH 7.5.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: 5'-Nucleotidase/5'-NT/CD73 (NT5E Produits)

Synonymes: CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein

Sujet:

Background: CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Synonym: 5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, CD73, NT5E, NT5, NTE

Poids moléculaire: 58.8 kDa

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process