CA12 Protein (His tag)

N° du produit ABIN7194556

Détail du produit

Antigène: CA12 (Carbonic Anhydrase 12 (CA12))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette CA12 protéine est marqué à la His tag.

Fonction: Recombinant Mouse Carbonic Anhydrase XII/CA12 Protein (His Tag)(Active)

Séquence: Met 1-Ser 301

Attributs du produit: A DNA sequence encoding the extracellular domain (Met 1-Ser 301) of mouse CA12 (NP_848483.2) precursor was expressed with a C-terminal polyhistidine tag.

Pureté: > 95 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.4 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CA12 (Carbonic Anhydrase 12 (CA12))

Autre désignation: Carbonic Anhydrase XII/CA12 (CA12 Produits)

Synonymes: CAXII Protein, HsT18816 Protein, CAR12 Protein, 2310047E01Rik Protein, AI314958 Protein, Ca12 Protein, ca12 Protein, carbonic anhydrase 12 Protein, carbonic anhydrase XII L homeolog Protein, CA12 Protein, Car12 Protein, ca12.L Protein

Sujet:

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

Synonym: Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; CA12; Carbonate dehydratase XII; CAXII;Car12

Poids moléculaire: 32.8 kDa

NCBI Accession: NP_848483