CSNK1G1 Protein (GST tag,His tag)

N° du produit ABIN7317070

Détail du produit

Antigène: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette CSNK1G1 protéine est marqué à la GST tag,His tag.

Fonction: Recombinant Human CSNK1G1/CKI-gamma 1 Protein (His & GST Tag)(Active)

Séquence: Met 1-Lys 422

Attributs du produit: A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Pureté: > 94 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 4 nmol/min/mg using casein as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Autre désignation: CSNK1G1/CKI-gamma 1 (CSNK1G1 Produits)

Synonymes: CSNK1G1 Protein, csnk1g2 Protein, zgc:101563 Protein, CK1gamma1 Protein, 9130020E21Rik Protein, casein kinase 1 gamma 1 Protein, casein kinase 1, gamma 1 Protein, casein kinase 1 gamma 1 S homeolog Protein, CSNK1G1 Protein, csnk1g1 Protein, csnk1g1.S Protein, Csnk1g1 Protein

Sujet:

Background: Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. The casein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine-threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53%-98% identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.

Synonym: CK1gamma1

Poids moléculaire: 76.3 kDa

NCBI Accession: NP_071331

Pathways: Signalisation Hedgehog