CADM3 Protein (His tag)

N° du produit ABIN7317622

Détail du produit

Antigène: CADM3 (Cell Adhesion Molecule 3 (CADM3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CADM3 protéine est marqué à la His tag.

Fonction: Recombinant Human CADM3 Protein (His Tag)(Active)

Séquence: Met 1-His 330

Attributs du produit: A DNA sequence encoding the human CADM3 isoform 2 (short isoform) (NP_001120645.1) extracellular domain (Met 1-His 330) was expressed, with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Human CADM3 immobilized (0.8 μg/ml, 100 μl/well) will mediate >30% C6 cell adhesion.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CADM3 (Cell Adhesion Molecule 3 (CADM3))

Autre désignation: CADM3 (CADM3 Produits)

Synonymes: BIgR Protein, IGSF4B Protein, NECL1 Protein, Necl-1 Protein, TSLL1 Protein, synCAM3 Protein, Igsf4b Protein, Necl1 Protein, SynCAM3 Protein, Tsll1 Protein, necl1 Protein, cell adhesion molecule 3 Protein, cell adhesion molecule 3 S homeolog Protein, CADM3 Protein, Cadm3 Protein, cadm3.S Protein

Sujet:

Background: Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. These proteins are typically transmembrane receptors and are composed of three domains: an intracellular domain that interacts with the cytoskeleton, a transmembrane domain, and an extracellular domain that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding). Cell adhesion molecule 3, also known as Immunoglobulin superfamily member 4B, CADM3, and NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Isoform 1 of CADM3 is expressed mainly in adult and fetal brain. Isoform 2 of CADM3 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum. CADM3 is involved in the cell-cell adhesion. It has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. The interaction with EPB41L1 may regulate structure or function of cell-cell junctions. CADM3 may act as a tumor suppressor in glioma and loss of it in glioma may be caused by histone deacetylation.

Synonym: Cell Adhesion Molecule 3, Brain Immunoglobulin Receptor, Immunoglobulin Superfamily Member 4B, IgSF4B, Nectin-Like Protein 1, NECL-1, Synaptic Cell Adhesion Molecule 3, SynCAM3, TSLC1-Like Protein 1, TSLL1, CADM3, IGSF4B, NECL1, SYNCAM3, TSLL1

Poids moléculaire: 35.1 kDa

NCBI Accession: NP_001120645

Pathways: Cell-Cell Junction Organization