CHEK2 Protein (His-GST)

N° du produit ABIN7320011

Cette protéine Recombinant CHEK2 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour CHEK2 Protein (His-GST) (ABIN7320011)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CHEK2 protéine est marqué à la His-GST.

Fonction: Recombinant Mouse CHK2/CHEK2 Protein (His & GST Tag)

Séquence: Mey 1-Leu 546

Attributs du produit: A DNA sequence encoding the mouse CHEK2 (Q9Z265) (Mey 1-Leu 546) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHK2/CHEK2

Sujet: CHK2,Cds1,HUCDS1,Rad53,In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire:

Calculated MW: 89.0 kDa

Observed MW: 90 kDa

UniProt: Q9Z265

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire