CHEK2 Protein (GST tag)

N° du produit ABIN7317121

Détail du produit

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette CHEK2 protéine est marqué à la GST tag.

Fonction: Recombinant Human CHK2/CHEK2 Protein (GST Tag)

Séquence: Met 1-Leu543

Attributs du produit: A DNA sequence encoding the human CHEK2 (NP_009125.1) (Met1-Leu543) was expressed with a GST tag at the N-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, 25 %glycerol, pH 7.5, 0.1 mM EDTA, 0.5 mM TCEP

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHK2/CHEK2 (CHEK2 Produits)

Synonymes: CDS1 Protein, CHK2 Protein, HuCds1 Protein, LFS2 Protein, PP1425 Protein, RAD53 Protein, hCds1 Protein, fa66f08 Protein, wu:fa66f08 Protein, zgc:55865 Protein, Cds1 Protein, HUCDS1 Protein, Rad53 Protein, Chk2 Protein, cds1 Protein, chek2 Protein, chk2 Protein, hucds1 Protein, lfs2 Protein, pp1425 Protein, rad53 Protein, checkpoint kinase 2 Protein, serine/threonine-protein kinase chk2 Protein, checkpoint kinase 2 L homeolog Protein, CHEK2 Protein, chek2 Protein, Chek2 Protein, MCYG_07308 Protein, chek2.L Protein

Sujet:

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CDS1;CHK2;hCds1;HuCds1;LFS2;PP1425;RAD53

Poids moléculaire: 88.1 kDa

NCBI Accession: NP_009125

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire