Cyclin E1 Protein (CCNE1) (His tag)

N° du produit ABIN7317727

Détail du produit

Antigène: Cyclin E1 (CCNE1)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Cyclin E1 protéine est marqué à la His tag.

Fonction: Recombinant Human CCNE1/Cyclin-E1 Protein (His Tag)(Active)

Séquence: Met 1-Ala 410

Attributs du produit: A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was expressed, with a polyhistidine tag at the N-terminus.

Pureté: > 85 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK4, The EC50 of biotinylated human CDK4 is 0.10-0.32 μg/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.5, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Cyclin E1 (CCNE1)

Autre désignation: CCNE1/Cyclin-E1 (CCNE1 Produits)

Synonymes: CCNE1 Protein, ccne Protein, cyclinE Protein, CCNE Protein, AW538188 Protein, CycE1 Protein, CYCLE Protein, Ccne Protein, cyce2 Protein, cycE3 Protein, cyce Protein, fa18e07 Protein, wu:fa18e07 Protein, cyclin E1 Protein, cyclin E1 L homeolog Protein, cyclin E1 S homeolog Protein, CCNE1 Protein, ccne1 Protein, Ccne1 Protein, ccne1.L Protein, ccne1.S Protein

Sujet:

Background: Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Synonym: CCNE

Poids moléculaire: 49.3 kDa

NCBI Accession: NP_001229

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases