FCN1 Protein (His tag)

N° du produit ABIN7317719

Détail du produit

Antigène: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FCN1 protéine est marqué à la His tag.

Fonction: Recombinant Human Ficolin-1/Ficolin-A/FCN1 Protein (His Tag)(Active)

Séquence: Met 1-Ala 326

Attributs du produit: A DNA sequence encoding the human FCN1 (NP_001994.2) precursor (Met 1-Ala 326) was expressed with a C-terminal polyhistidine tag.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.1. Immobilized FCN1 at 10 μg/ml can bind biotinylated recombinant human Fetuin-A with a linear range of 16-2000 ng/ml.2. Immobilized FCN1 at 10 μg/ml can bind biotinylated recombinant mouse Fetuin-A with a linear range of 16-2000 ng/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Autre désignation: Ficolin-1/Ficolin-A/FCN1 (FCN1 Produits)

Synonymes: FCNM Protein, FCNB Protein, FCN2 Protein, FCN1 Protein, ficolin 1 Protein, ficolin (collagen/fibrinogen domain containing) 1 Protein, ficolin-1 Protein, FCN1 Protein, Fcn1 Protein, LOC100069029 Protein

Sujet:

Background: Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin, H-Ficolin and M-Ficolin (also referred to as Ficolin-2, -3 and -1, respectively). They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases, autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent, the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs), and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes.

Synonym: FCN1,FCNA,FCNM

Poids moléculaire: 33.6 kDa

NCBI Accession: NP_001994

Pathways: Système du Complément