MUC16 Protein (His tag)

N° du produit ABIN7319764

Détail du produit

Antigène: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette MUC16 protéine est marqué à la His tag.

Fonction: Recombinant Human CA125/MUC16 (C-6His)

Séquence: Gly12660-Met12923

Attributs du produit: Recombinant Human Mucin-16 is produced by our Mammalian expression system and the target gene encoding Gly12660-Met12923 is expressed with a 6His tag at the C-terminus.

Pureté: >95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Mesothelin(Cat#CP52) at 10μg/ml (100 μl/well) can bind Human CA125-His (Cat#CW57).The ED50 of Human CA125-His (Cat#CW57) is 0.6μg/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 100 mM Glycine, pH 7.5.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Autre désignation: CA125/MUC16 (CA125 Produits)

Synonymes: CA125 Protein, 1110008I14Rik Protein, Ca125 Protein, Gm1480 Protein, RGD1308422 Protein, MUC16 Protein, mucin 16, cell surface associated Protein, mucin 16 Protein, mucin-16 Protein, MUC16 Protein, Muc16 Protein, LOC476718 Protein, LOC712735 Protein

Sujet:

Background: MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers. The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity. MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells. MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion. MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy. In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome.

Synonym: CA125 ovarian cancer antigen, CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, cell surface associated, mucin-16

Poids moléculaire: 29.2 kDa

UniProt: Q8WXI7